On recovery, swabs were removed from SDPs and plated for lawn growth. Department of Microbiology and Immunology, the University of Melbourne, Parkville, Victoria, Australia. Our data demonstrate that S. pneumoniae remain suitably viable in SDPs for up to 14 days at room temperature, or 28 days at 4° C. Similarly, most of the other bacterial species tested remained viable after 28 days storage at room temperature. For all species, except N. gonorrhoeae and H. influenzae, full lawns (TNTC) were recovered at all time points. However, portions of lawn culture were used as the inocula as a practical alternative to a defined CFU, given that our pilot data found viable counts from quarter lawns were comparable for the initial S. pneumoniae isolates tested (mean 2.43 x 108 CFU (95% CI: 7.04 x 107, 4.15 x 108)). Casey L. Pell, Melanie J. Williams, [...], and Catherine Satzke. Silica Gel Dessicant Packets are invaluable for keeping things dry. What type of container or bag is being used for packaging? At 28 days, all inocula showed reduced recovery of satisfactory growth (data not shown), and half-lawns yielded more recovery of satisfactory growth compared with quarter-lawns (P =0.0489). Dry & Dry 2 Gram [100 Packets] Premium Pure Silica Gel Packets Desiccant Dehumidifier Silica Gel Packs - Food Safe Rechargeable Silica Packets Silica Gel Packs 4.8 out of 5 stars 928 $7.99 $ 7 . There was no significant difference between comparisons with cotton swabs (382.7 (95% CI: 66.24, 699.2)) or when moistened swabs were analyzed separately (see Materials and Methods, data not shown). Pilot studies indicated that higher inocula may increase storage limits for H. influenzae; this could be further investigated in future studies. c TNTC, >1000 CFU; Heavy, 100-1000 CFU; Moderate, 20-99 CFU; Light, <20 CFU; and No growth, 0 CFU. Silica gel desiccant is designed to remove excess moisture from enclosed spaces. et al. Here at Aquadry, we sell bulk silica gel packets ranging from 1g to 500g capacity. b Satisfactory growth, ≥ 100 CFU; unsatisfactory growth, <100 CFU. (2011), Ortika BD, Habib M, Dunne EM, Porter BD, Satzke C (2013), Production of latex agglutination reagents for pneumococcal serotyping, A comparative study of smooth and rough pneumococcus colonies, Wasas AD, Huebner RE, De Blanche M, Klugman KP (1998), Mason CK, Goldsmith CE, Moore JE, McCarron P, Leggett P SDP transport does not require specialized equipment or maintenance of temperature during transport, the costs of which can be unattainable for low-income countries. Neisseria et al. Where to Use Silica Gel Packets 99 (CDN$ 6.00/Item) Further investigations could assess suitability of longer storage times at 4° C. Unfavorable storage conditions may influence colony morphology and the ability to serotype S. pneumoniae isolates [14]. Bacterial isolates are often transported between laboratories for research and diagnostic purposes. (2006), Recovering streptococci from the throat, a practical alternative to direct plating in remote tropical communities, Popovic T, Kim C, Schmink S, Ajello G (1998), Evaluation of silica gel packages for transport of, Improved dry-swab transportation for streptococcal specimens, Joshi HH, Gertz RE Jr., da Gloria Carvalho M, Beall BW (2008), Use of silica desiccant packets for specimen storage and transport to evaluate pneumococcal nasopharyngeal carriage among Nepalese children, Castillo D, Harcourt B, Hatcher C, Jackson M, Katz L Food Grade Silica Gel Desiccant Packets for Food Storage These one-gram silica-gel desiccant packets are perfect for spice jars, but use them whenever you want to … [5–9]. The optimized protocol was then further tested with 49 additional S. pneumoniae isolates and nine other clinically relevant bacterial species. No growth was recovered for isolates representing serotypes 14, 23A, 33F and 33C after 28 days of storage. Oxygen absorbers are little packets that contain a mixture of iron powder, natural zeolite powder, salt, water, and FDA-approved Tyvek paper. What is the volume or area of the container (Formula: L x W … a Before storage and after recovery, S. pneumoniae isolates were serotyped using a combination of commercially available reagents (Denka Seiken Co. Ltd, Japan) and latex agglutination reagents prepared in-house from Statens Serum Institut antisera [13]. Variants were most commonly observed from swabs stored for 28 days at room temperature, and represented a small portion of total growth recovered. For the provision of isolates we thank Fiona Russell and the Fiji Pneumococcal Project team; Samir Saha, staff of the Child Health Research Foundation, Dhaka Shishu Hospital, and families who participated in the Pneumococcal carriage project; and the Department of Microbiology, Laboratory Services, Royal Children’s Hospital, Melbourne. 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